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Ambler is a city in Northwest Arctic Borough, Alaska, United States. As of the 2020 census, Ambler had a population of 274. The city is located in the large Iñupiaq language speaking region of Alaska, and the local dialect is known as the Ambler dialect. As of 1999, over 91% of the community speaks and understands the language, with many young children actively learning the language in school. It has important relationships with the "hub" city of Kotzebue, Alaska and has important relationships with Maniilaq Health Association.