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Burlington is a city in Alamance and Guilford counties in the U.S. state of North Carolina. It is the principal city of the Burlington, NC Metropolitan Statistical Area, which encompasses all of Alamance County, in which most of the city is located, and is a part of the Greensboro–Winston-Salem–High Point, NC Combined Statistical Area. The population was 57,303 at the 2020 census, which makes Burlington the 18th-most populous city in North Carolina.