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Florence is a city in and the county seat of Lauderdale County, Alabama, United States, in the state's northwestern corner, and had a population of 40,184 in the 2020 census. Florence is located along the Tennessee River and is home to the University of North Alabama, the oldest public college in the state. Florence is located about 70 miles (110 km) west of Huntsville, Alabama, via US-72, and about 115 miles (185 km) northwest of Birmingham, Alabama.