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Harbor Island is a small residential barrier island located 14 miles (22 km) east of Beaufort, South Carolina. It is one of the Carolina Sea Islands. Most of the island is tidal marsh, though approximately 800 acres (3.2 km2) of upland acreage exists on the northeastern portions. The residential portion of the island was listed as a census-designated place in the 2020 census, with a population of 209. The island is served by U.S. Route 21, the only vehicular access route to the island and other nearby islands.